Catmint (Nepeta nuda L.) phylogenetics defined by nuclear and chloroplast DNA barcodes

Nepeta nuda L. (Lamiaceae) is a medicinal plant with wide distribution in Europa and Asia. In Bulgaria, N. also known as “naked (or hairless) catmint”, which likely refers to the naked or sparse short hairy stem leaves. This study aims generate DNA barcodes for precise genetic discrimination determination of phylogenetic position among other species. To achieve this goal, we applied barcoding technique based on conserved nuclear (internal transcribed spacer/ITS) chloroplast ( rbcL , matK trnH ) regions. The generated barcode sequences were submitted Barcode Life Data System (BOLD) database (https://www.boldsystems.org; accession number BUL002-22). obtained corresponding species available BOLD NCBI utilized taxonomic classification. ITS-derived most enriched database, fragments matching 47 selected construction tree. results show that clustered subclade together sheilae deflersiana isaurica congesta heliotropifolia schiraziana cataria . information was retrieved corresponded 15 10 different species, respectively. For only 6 found. Тhe three markers highlighted close relation italica tuberosa grandiflora hemsleyana conclusion, suggest system could assist its accurate characterization.